Evaluation of the constituent compounds, antioxidant, anticancer, and antimicrobial potential of Prangos ferulacea plant extract and its effect on Listeria monocytogenes virulence gene expression

Prangos ferulacea plant is very popular in Iran due to its unique properties treating diseases and special flavor. To check the characteristics of this plant, first, extract was extracted using maceration method. Its chemical composition investigated high-performance liquid chromatography (HPLC) that p-coumaric identified as main compound, Fourier-transform infrared spectroscopy (FTIR) showed presence functional groups related phenolic, flavonoid, tannins, carboxylic acids such caffeic acid coumaric composition. Total phenol content (TPC), total flavonoid (TFC), beta-carotene were equal 202.04 ± 5.46 mg gallic equivalent (GAE)/g dry weight, 1,909.46 13 μg quercetin (QE)/g 2.91 mg/100 g. The antioxidant property evaluated 2,2-Diphenyl-1-picrylhydrazyl (DPPH) 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic (ABTS) free radical scavenging ferric reducing power assay (FRAP). According IC50 obtained for DDPH (274 7.2 μg/mL) ABTS (120.45 9.6 FRAP values [1.92 0.05 ascorbic (AAE)/g extract], had high properties. Cytotoxicity against survival HT 29 cells 82.15 0.02 μg/mL. antimicrobial calculated disk diffusion agar (DDA), well (WDA), minimum inhibitory concentration (MIC), bactericidal (MBC). Listeria monocytogenes has highest sensitivity inhibition zone based on DDA WDA method with an MIC MBC 16 128 mg/mL least resistance. morphology change L. strain proved through scanning electron microscope (SEM) confocal laser microscopy (CLSM). caused a significant reduction transcription genes involved film formation ability . results fully prove practical pragmatic P.